Laccase Enzyme from Trametes versicolor with a High Decolorizing Ability on Malachite Green

Laccases enzyme provide a promising future in terms of its use for the treatment of Malachite green (MG), which is highly toxic residue for aquatic animals. The objectives of this study were the production, purification and characterization of a laccase from Trametes versicolor 50001 with a degrading ability on malachite green. Trametes versicolor 50001 was used to produce laccase enzyme under liquid-state fermentation. The maximum enzyme activity was of 1071 ± 21 U l-1 after 9 days of fermentation. The enzyme was purified 31-fold by employing DEAE-Sepharose FF chromatography with a specific activity of 165.5 U mg-1 and a molecular mass about 50 60 kDa. The optimal pH and temperature were 2 and 35°C, respectively. Decolorization efficiency of laccase was evaluated against malachite green and the decolorization rate was 95.67% without added any mediator after 12h of treatment. The data suggested that the laccase from Trametes versicolor might has a great potential to decolorize malachite green in highly toxic residue.